Turkish Journal of Biology
DOI
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Abstract
We report here the development of hybrid cells producing monoclonal antibodies specific for two different steroid hormones with mixed immunization using hybridoma technology. BALB/c mice were immunized with a mixture of three steroid antigens: progesterone, estradiole and testosterone linked to bovine serum albumine. These mice were used for fusion. In the two fusion experiments, ELISA tests showed that among 645 wells only 2 hybrids reacted with progesterone (MAM 3C2, MAM 3E3) and one of them reacted specifically with estradiol (MAM 7H7). Three types of monoclonal antibodies were detected: IgG1, IgG2a and IgG2b and the affinities of these antibodies were found to be 10 -8 -10 -9 M. Monoclonal antibodies did not cross react with the other steroid hormones.
Keywords
Immunodiagnosis, hybrid cell, monoclonal antibody, steroid hormones, ELISA.
First Page
697
Last Page
706
Recommended Citation
YÜCEL, FATMA ŞAHİNGÖZ and ÇIRAKOĞLU, BEYAZIT (2000) "Production of Monoclonal Antibodies Specific forProgesterone, Estradiole by Simultaneous Injectionof Different Steroids," Turkish Journal of Biology: Vol. 24: No. 4, Article 2. Available at: https://journals.tubitak.gov.tr/biology/vol24/iss4/2
