Turkish Journal of Biology
Abstract
We report here the development of hybrid cells producing monoclonal antibodies specific for two different steroid hormones with mixed immunization using hybridoma technology. BALB/c mice were immunized with a mixture of three steroid antigens: progesterone, estradiole and testosterone linked to bovine serum albumine. These mice were used for fusion. In the two fusion experiments, ELISA tests showed that among 645 wells only 2 hybrids reacted with progesterone (MAM 3C2, MAM 3E3) and one of them reacted specifically with estradiol (MAM 7H7). Three types of monoclonal antibodies were detected: IgG1, IgG2a and IgG2b and the affinities of these antibodies were found to be 10 -8 -10 -9 M. Monoclonal antibodies did not cross react with the other steroid hormones.
DOI
-
Keywords
Immunodiagnosis, hybrid cell, monoclonal antibody, steroid hormones, ELISA.
First Page
697
Last Page
706
Recommended Citation
YÜCEL, F. Ş, & ÇIRAKOĞLU, B (2000). Production of Monoclonal Antibodies Specific forProgesterone, Estradiole by Simultaneous Injectionof Different Steroids. Turkish Journal of Biology 24 (4): 697-706. https://doi.org/-
