Turkish Journal of Biology




Olive oil mill effluent (OOME) is one of the major pollutants produced by the agricultural industry in Mediterranean countries. The olive oil mills in these countries generate about 2-3 million tonnes of effluent annually. OOME is a black liquor containing a water-soluble fraction of ripe olives and water that is used in the process of olive oil extraction. This waste has a high mineral content and a chemical oxygen demand of 25.000-100.000 mg/l. depending on the olive oil extraction process used. This study presents a process for biosurfactant as rhamnolipid production by Pseudomonas sp. from olive oil mill effluent (OMME). For this purpose, some biosurfactant-producing strains were assayed and several strains of Pseudomonas sp. were able to grow on OOME as the sole carbon source and accumulate rhamnolipids. Samples of OOME used as a medium of production were diluted and it was only necesarry to add NaNO 3 (2.5 g/l) to the medium. The conversion yield was 0.875 g of rhamnolipid per litre of substrate (OOME). The COD of the OOME was reduced by 50-70% in 72 h.


Olive Oil Mill Effluent (OOME) Pseudomonas sp., Biosurfactant, Rhamnolipid.

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