Turkish Journal of Biology
Abstract
The transition of a quiescent plant embryo into a vigorously growing embryo revealed some nascent synthesis of gene products, among them germin has been characterized. Germin is a water soluble homopentameric glycoprotein whose synthesis and accumulation appear to be closely related to early wheat embryo development. Germin synthesis was monitored by employing an anti-germin serum which had been pre-adsorbed with wheat extract and optimized in order to decrease nonspecific binding. Germin accumulation was shown to occur in germinating embryos from 16h up to 6 day post-imbibition. Germin as a rare protein in wheat seedlings was detected as a dark band on X-rays in amounts as small as 20 µl protein extracts. Neither germin in mature wheat plant organs nor germinated embryos were detected prior to 16 hours of imbibition.
DOI
-
Keywords
Early Wheat development, Germin, Western blotting.
First Page
271
Last Page
278
Recommended Citation
ÇALIŞKAN, MAHMUT and CUMING, ANDREW C. (2000) "Monitoring of Germin Synthesis by Pre-AbsorbedAnti-Germin Serum," Turkish Journal of Biology: Vol. 24: No. 2, Article 9. Available at: https://journals.tubitak.gov.tr/biology/vol24/iss2/9
