Changes in Liver Cytoplasmic Glutathione-S-Transferase and MicrosomalNitrosodimethylamine Demethylase Activities in Chicks ObtainedFollowing Daminozide Treatment

Authors

YAVUZ SİLİĞ
V. KENAN ÇELİK
ATİLLA ATALAY

Abstract

In this study, microsomal nitrosodimethylamine demethylase (NDMAd) and cytoplasmic glutathione-s-tarnsferase (GST) activities in the livers of chicks obtained from the LD 10 (3 mg/ml) and LD 30 (9 mg/ml) doses of daminozide (Succinic acid 2,2-Dimethylhydrazide) injected Ross-Pm-3 fertilized chicken eggs were investigated. Spectrophotometric determination of NDMAd activity was performed at 412 nm by the detection of yellow coloured compound formed as a product of the reaction of formaldehyde with Nash reagent. No significant increase was observed in NDMAd activity (P>0.05) of the group treated with LD 10 dose although NDMAd activity was found to be significantly increased (P

DOI

-

Keywords

Daminozide, Nitrosodimethylamine Demethylase, Cytochrome P-450IIE1 Glutathione-S-Transferase.

First Page

119

Last Page

126

Recommended Citation

SİLİĞ, YAVUZ; ÇELİK, V. KENAN; and ATALAY, ATİLLA (2000) "Changes in Liver Cytoplasmic Glutathione-S-Transferase and MicrosomalNitrosodimethylamine Demethylase Activities in Chicks ObtainedFollowing Daminozide Treatment," Turkish Journal of Biology: Vol. 24: No. 1, Article 10. Available at: https://journals.tubitak.gov.tr/biology/vol24/iss1/10