Turkish Journal of Biology
DOI
-
Abstract
A procedure employed in the purification of anthranilate synhetase of Escherichia coli SJHI is described. The purified anthranilate synthetase appeared to be homogeneous when examined with poliacrylamide gel electrophoresis. Phenly-sepharose CL-4B and Blue dye sepharose were used for purification. A positive correlation was found between purification and ammonium sulfate especially using concentrations greater than 10%. A positive effect 2-Mercapto-ethanol on the enzyme stability was also determined.
Keywords
Purification, Anthranilate synthetase, Escherichia coli SJHI.
First Page
169
Last Page
176
Recommended Citation
ARISOY, MÜNEVVER (1999) "Anthranilate Synthetase From Escherichia coli SJHI:Purification and Some Properties," Turkish Journal of Biology: Vol. 23: No. 2, Article 5. Available at: https://journals.tubitak.gov.tr/biology/vol23/iss2/5
