Turkish Journal of Biology
Abstract
A procedure employed in the purification of anthranilate synhetase of Escherichia coli SJHI is described. The purified anthranilate synthetase appeared to be homogeneous when examined with poliacrylamide gel electrophoresis. Phenly-sepharose CL-4B and Blue dye sepharose were used for purification. A positive correlation was found between purification and ammonium sulfate especially using concentrations greater than 10%. A positive effect 2-Mercapto-ethanol on the enzyme stability was also determined.
DOI
-
Keywords
Purification, Anthranilate synthetase, Escherichia coli SJHI.
First Page
169
Last Page
176
Recommended Citation
ARISOY, M (1999). Anthranilate Synthetase From Escherichia coli SJHI:Purification and Some Properties. Turkish Journal of Biology 23 (2): 169-176. https://doi.org/-
