Turkish Journal of Biology
DOI
-
Abstract
The levels of intracellular calcium [Ca 2+ ]i were examined in CO25 myoblast cells bearing a mutated human N-ras oncogene, after stimulation by thapsigargin (ThG), bombesin and epidermal growth factor (EGF). Thapsigargin induced a [Ca 2+ ]i rise mostly from intracellular calcium pools, and a small Ca 2+ entry into cytoplasm via the plasma membrane channel. In both ras-induced and differentiated CO25 cells, [Ca 2+ ]i increase showed a similar pattern of events after addition of ThG. The addition of EGF and bombesin to both ras-induced and differentiated cells did not induce Ca 2+ mobilization, suggesting that the cells may not have receptors for the ligands, or desensitization of the receptors may have occured by a different signalling pathway such as stimulation of protein kinase C activity.
Keywords
N-ras, myoblast, [Ca 2+ ]i, thapsigargin, EGF, bombesin.
First Page
159
Last Page
168
Recommended Citation
ZEYTİNOĞLU, HÜLYA and GIBSON, IAN (1999) "Cytosolic Ca 2+ levels in CO25 myoblast cells bearinga human N-ras oncogene after stimulation by thap-sigarginand growth factors," Turkish Journal of Biology: Vol. 23: No. 2, Article 4. Available at: https://journals.tubitak.gov.tr/biology/vol23/iss2/4
