An intracellular, thermostable a-galactosidase (a-D-galactoside galactohydrolase EC 126.96.36.199.) was produced from a strain of Bacillus stearothermophilus var. calidolactis C953. It was found that the presence of NH 4 Cl in the fermentation medium affected microbial growth and that optimum enzyme production occurred with peptone in various sources of nitrogen where NH 4 Cl was used as the main inorganic nitrogen source. After peptone, in decreasing order of enzyme productivity, came tryptone, soybean meal, soya-neutralized peptone and casamino acid. It was determined that, in various carbon sources, raffinose produced the highest a-galactosidase activity, followed by melibiose, D-galactose and molasses, and that in the medium with soybean meal extract the second highest level of activity occurred. Soybean oligosaccharides (raffinose-like sugars), because of their present low commercial value, could be of pratical use in the production of a-galactosidase.
a-Galactosidase, Microbial enzyme production, Bacillus stearothermophilus
KOCABAŞ, E. Esin and DİZBAY, Mücahit (1999) "Production of \alpha -Galactosidase usingBacillus stearothermophilusvar. caldiloactis C953," Turkish Journal of Biology: Vol. 23: No. 1, Article 6. Available at: https://journals.tubitak.gov.tr/biology/vol23/iss1/6