Purification and Characterization of Pediocin F, ABacteriocin Produced By Pediococcus acidilacticiF

Authors

Özlem OSMANAGAOĞLU
Ufuk GÜNDÜZ
Yavuz BEYATLI
Cumhur ÇÖKMÜŞ

Abstract

In this work, Pediococcus acidilactici F isolated from fermented sausage was reported to produce an extracellular antimicrobial peptide, termed Pediocin F, that was protein in nature and effec-tive against many bacteria associated with food spoilage and food related health hazards. Pediocin F is reported here to be sensitive to proteolytic enzymes, resistant to heat and organic solvents, and active over a wide range of pH. We have also reported that the extracellular antimicrobial protein pro-duced by P. acidilactici F was really a small peptide. Coomassie-blue stained SDS-PAGE technique allowed us to estimate the molecular weight of this small peptide to be about 4460 dalton.

DOI

-

Keywords

Pediococcus acidilactici, Pediocin, characterization, SDS-PAGE

First Page

217

Last Page

228

Recommended Citation

OSMANAGAOĞLU, Özlem; GÜNDÜZ, Ufuk; BEYATLI, Yavuz; and ÇÖKMÜŞ, Cumhur (1998) "Purification and Characterization of Pediocin F, ABacteriocin Produced By Pediococcus acidilacticiF," Turkish Journal of Biology: Vol. 22: No. 2, Article 10. Available at: https://journals.tubitak.gov.tr/biology/vol22/iss2/10