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Turkish Journal of Biology

Production of Chimeric Mouse by Injection of Embryonic Stem (ES) Cells to Morula from An Outbred Mouse Strain

Sezen ARAT

Abstract

The aim this study was to determine the capability of embryos from an outbred OF1 mouse strain to form ES cell-embryo chimeras with morulae injection. When the R1 ES cell line was routinely cultured on mitotically inactivated feeder cells in a culture medium supplemented with Leukemia Inhibitory Factor (LIF), the colonies maintained undifferentiated ideal ES cell morphology. When ES cells were injected into 55 eight-cell embryos, 73% of the embryos combined with ES cells. 31% of the embryos transferred to recipients developed to term. 27% of offspring were chimeric. ES cells contributed about 90% of the coat in the chimeras. This study demonstrates. that high frequencies of chimerism can be obtained by injection of ES cells to morula from the OF1 outbred strain.

DOI

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Keywords

Mouse, ES cells, morula injection, chimera

First Page

431

Last Page

439

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