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Turkish Journal of Biology

Article Title

The Use of Polymerase Chain Reaction in Brassica napus Transformation Studies

Authors

Kenan TURGUT

DOI

-

Abstract

The polymerase chain reaction (PCR) was used to follow the inheritance of the transferred DNA through the T2 and 3 generations of transformed Brassica napus plants. Two oligonucleotide primers (A9 5'-126 and 3'-240) were designed on the A9 cDNA sequence and used with plasmid primers (M13-20 and RSPL). In the T2 generation (progeny of initial transformants), M13-20+A9 5'-126 primers, and in the T3 generation, RSPL+A9 3'-240 primers were used and gave 0.9 kb and 1.3 kb fragments, respectively. The results demonstrated that polymerase chain reaction was a useful and reliable technique for determination of the transformed plants.

First Page

115

Last Page

120

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