Influence of a new nanoensemble of graphene oxide and indole-3-acetic acid on the morphophysiological, biochemical, and ultrastructural characteristics of upland cotton (Gossypium hirsutum L.)

Authors

• NURLAN AMRAHOVFollow
• ULVIYYA HASANOVAFollow
• ALIYEVA GUNELFollow
• AGHAZADA GONCHAFollow
• FUAD RZAYEVFollow
• ELDAR GASIMOVFollow
• ALIYEVA SOLMAZFollow
• LALA GAHRAMANLIFollow
• ELVIN IBRAHIMLIFollow
• RUHANGIZ MAMMADOVAFollow
• ZAREMA GAKHRAMANOVAFollow
• CEM BAYRAMFollow
• GONCHA EYVAZOVAFollow
• SİBEL HASANOVAFollow
• ZIYADDIN MAMMADOVFollow

Abstract

​The exogenous application of phytohormones plays a crucial role in the activation of adaptive mechanisms in plants under both normal and stress conditions. Therefore, the present study investigates the synthesis, characterization, and application of nanoensembles based on indole-3-acetic acid (IAA) and graphene oxide (GO) nanolayers. The surface of GO synthesized by the modified Hummer method was noncovalently functionalized with IAA molecules. The structure and morphological properties of the prepared materials were analyzed by Fourier transform infrared spectroscopy, X-ray diffraction, UV-Vis spectroscopy, and transmission electron microscopy. The IAA and IAA+GO nanoensemble enhanced the morphological parameters, lateral root formation, catalase and peroxidase activities, and nitric oxide production of upland cotton (Gossypium hirsutum L.). It also promoted stem elongation more effectively than pure IAA. Additionally, it significantly boosted polyphenol oxidase activity in leaf tissues. However, IAA+GO reduced the primary root length compared to IAA alone at the same concentration. In summary, the IAA+GO nanoensemble offers a promising approach for enhancing plant growth and development. Its impact on stem elongation, lateral root development, and the activation of enzymatic defense systems points to its potential application in agricultural practices aimed at improving crop productivity and stress resilience. Light and ultrastructural microscopy demonstrated that both IAA and IAA+GO induced cellular gigantism across all examined vegetative organs of cotton plants. Overall, the IAA+GO nanoensemble exhibits a synergistic effect on plant stem elongation and antioxidant defense mechanisms, enhancing nitric oxide-mediated signaling without inducing osmotic stress.

Author ORCID Identifier

NURLAN AMRAHOV: 0000-0002-6916-7672

ULVIYYA HASANOVA: 0000-0003-1502-4227

ALIYEVA GUNEL: 0000-0002-6995-8589

AGHAZADA GONCHA: 0009-0008-1254-2399

FUAD RZAYEV: 0000-0002-8128-1101

ELDAR GASIMOV: 0000-0002-5104-4260

ALIYEVA SOLMAZ: 0000-0003-4345-526X

LALA GAHRAMANLI: 0000-0002-3799-8578

ELVIN IBRAHIMLI: 0000-0003-2930-3833

RUHANGIZ MAMMADOVA: 0000-0003-0776-0937

ZAREMA GAKHRAMANOVA: 0000-0002-5018-7198

CEM BAYRAM: 0000-0001-8717-4668

GONCHA EYVAZOVA : 0000-0003-4560-3756

SİBEL HASANOVA: 0009-0009-5239-3081

ZIYADDIN MAMMADOV: 0000-0001-8009-194X

DOI

10.55730/1300-008X.2908

Keywords

Nanoensembles, graphene oxide, indole-3-acetic acid, antioxidant enzymes, transmission electron microscopy, upland cotton

First Page

318

Last Page

340

Publisher

The Scientific and Technological Research Council of Türkiye (TÜBİTAK)

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Recommended Citation

AMRAHOV, N, HASANOVA, U, GUNEL, A, GONCHA, A, RZAYEV, F, GASIMOV, E, SOLMAZ, A, GAHRAMANLI, L, IBRAHIMLI, E, MAMMADOVA, R, GAKHRAMANOVA, Z, BAYRAM, C, EYVAZOVA, G, HASANOVA, S, & MAMMADOV, Z (2026). Influence of a new nanoensemble of graphene oxide and indole-3-acetic acid on the morphophysiological, biochemical, and ultrastructural characteristics of upland cotton (Gossypium hirsutum L.). Turkish Journal of Botany 50 (4): 318-340. https://doi.org/10.55730/1300-008X.2908