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Turkish Journal of Botany

Author ORCID Identifier

MUMTAZ SAAND: 0009-0000-3721-9039

MARINA MEMON: 0009-0001-0932-4525

AMEER', 'AMIR', 'QURBAN MİRBAHAR', 'MAGSI: 0000-0002-0798-647X

ATHAR BABAR: 0009-0007-3695-8797

SAHIB GHANGHRO: 0009-0002-8387-8021

DOI

10.55730/1300-008X.2813

Abstract

Cyclic nucleotide-gated ion channel (CNGC) is one of the important calcium conducting channels and involves in numerous biological functions such as plant development, phytohormone responses, plant-pathogen interactions. Nevertheless, identification of CNGC gene family has yet not been carried out in date palm crop. In this study, genome-wide identification, and pharmacological analysis of date CNGCs was conducted against fungal pathogens. The 26 PdCNGC genes were identified by using extensive bioinformatics tools. Gene ontology (GO) analysis showed that signal transduction, transmembrane transport and ion binding proteins were at the top in biological process and molecular functions of PdCNGCs. Protein-protein interaction of PdCNGCs and their patterners showed complex network that may involve in nitric oxide and calcium ion mediated signal transduction and plant-pathogen interactions in response to stresses. The compressive phylogenetic analysis revealed that group IV is ancient as entire CNGCs of non-vascular (moss) and vascular non-flowering (Selaginella) plants were clustered with group IVa and IVb. Furthermore, the pharmacological analysis manifested that infiltration of cGMP and cAMP (CNG channel activators) may enhance susceptibility against Aspergillus ssp. in leave and stem of date palm respectively. While the treatment of LaCl3 (calcium channel blocker) also displayed the susceptibility to Fusarium in date fruit. These results suggest that role of CNGC and calcium ion may be negatively regulated in terms of disease resistance in date palm leaves and fruit against fungal pathogens. However, the functional analysis through gene silencing may confirm the role of pharmacological effectors for calcium mediate CNGC-activation in date palm in response to biotic stress in future. Finally, our study would be the basis for functional analysis of date palm CNGCs and their molecular mechanisms in nexus of pharmacological effect in plant diseases resistance.

Keywords

Date palm, cyclic nucleotide-gated ion channel, phylogeny, gene ontology, pharmacological assay, fungi

First Page

249

Last Page

261

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

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