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Turkish Journal of Botany

Author ORCID Identifier

YURIY KOLUPAEV: 0000-0001-7151-906X

IVAN SHAKHOV: 0009-0009-6378-9866

ALEXANDER', 'LIANA KOKOREV', 'RELINA: 0000-0002-0429-0514

ALLA DYACHENKO: 0000-0002-9020-3041

ALEXANDER DMITRIEV: 0000-0003-2268-9104

DOI

10.55730/1300-008X.2812

Abstract

Gamma-aminobutyric acid (GABA) is currently considered to be an important stress metabolite with regulatory functions in plants. Exogenous GABA is known to be able to enhance the tolerance of different plant species to drought and salt stress. However, its effects on the functioning of stress-protective systems in triticale, an intergeneric wheat-rye hybrid with specific adaptation strategies to abiotic stresses, remained completely unstudied. We studied the effects of exogenous GABA (0.5 mM) on the growth as well as on the antioxidant and osmoprotective systems of triticale seedlings (cv. 'Rarytet') under simulated drought (15% PEG 6000), salt stress (100 mM NaCl), or a combination of these factors. GABA mitigated the growth-inhibitory effects of drought approximately by 15% and of salt stress approximately by 23%, but the most noticeable effect of GABA was seen on biomass accumulation upon combined stress: it abated the biomass accumulation inhibition caused by drought-salt stress combination by 67%. GABA also increased the water contents in seedling tissues subjected to each stressor separately or to their combination. GABA considerably reduced drought- and salt-triggered enhancement of ROS generation and lipid peroxidation. GABA increased the activities of antioxidant enzymes such as superoxide dismutase, catalase, and guaiacol peroxidase under physiologically normal conditions, under the influence of PEG 6000 or NaCl, and upon drought-salt stress combination GABA prevented a reduction in the activities of these enzymes. Under normal and stressful conditions, exogenous GABA also modulated the contents of proline, sugars, phenolic compounds, and anthocyanins in seedlings. We concluded that the anti-stress systems of triticale were highly susceptible to GABA and that it was possible to use GABA in order to enhance the tolerance of this species to osmotic stresses, especially to the combined impact of drought and salinity.

Keywords

× Triticosecale, antioxidant system, drought, gamma-aminobutyric acid, osmolytes, salt stress

First Page

235

Last Page

248

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

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