Turkish Journal of Botany
DOI
-
Abstract
In the present work, we first describe the transfer of a novel gene, designated 5W, into the LBA4404 Agrobacterium tumefaciens strain using pPCV91 plasmid through a triparental mating system. The 5W gene was recently isolated from macadamia, a native nut tree species of Australia, and has been shown to have an antimicrobial effect against certain fungi in vitro. Secondly, this gene was tranferred into tobacco (Nicotiana tabacum cv. Xhantii) using leaf explants to develop a system which could then be used for transferring this antimicrobial peptide into sunflower cultivars.
Keywords
Macademia, tobacco, triparental mating, gene transfer, leaf explants
First Page
169
Last Page
175
Recommended Citation
GÜREL, EKREM (2001) "Insertion of an Antimicrobial Gene into Agrobacterium and its Further Use in Transforming Tobacco," Turkish Journal of Botany: Vol. 25: No. 4, Article 1. Available at: https://journals.tubitak.gov.tr/botany/vol25/iss4/1
