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Turkish Journal of Biology

Abstract

Background/aim: Collagen-derived peptides have shown potential in promoting hair growth, yet their effects on catagen-inducing factors remain unexplored. In this study, the hair growth-promoting effects and molecular mechanisms of Collagen-Tripep20 (CTP), a collagen tripeptide preparation containing 3.2% Gly-Pro-Hyp (GPH), were explored, with a specific focus on fibroblast growth factor 5 (FGF-5) regulation.

Materials and methods: Female C3H/HeJ mice were randomly assigned to four groups (n = 10/group): control, CTP-LOW (400 mg/ kg/day), CTP-HIGH (800 mg/kg/day), and GPH (25.6 mg/kg/day). Following dorsal depilation, test substances were administered dailyvia oral gavage for 7 weeks. Hair regrowth was assessed by visual scoring, histological analysis, and quantitative RT-PCR of dorsal skin tissues.

Results: CTP-HIGH significantly accelerated telogen-to-anagen transition, with elevated hair growth scores at weeks 5 and 6 (p < 0.01 and p < 0.05, respectively). Histological analysis confirmed a significant increase in hair follicle length in the CTP-HIGH group (p < 0.001). Both CTP and GPH upregulated the mRNA expression of anagen-promoting markers, including VEGF and FGF-7. Notably, GPH significantly downregulated FGF-5 mRNA to 0.86 ± 0.11-fold relative to the control (p < 0.05), whereas CTP-HIGH showed asimilar but nonsignificant trend (0.87 ± 0.07-fold).

Conclusion: CTP promotes anagen induction through upregulation of growth-related factors, while its bioactive component GPH additionally functions as a transcriptional suppressor of FGF-5, providing a dual-mechanistic rationale for collagen-derived tripeptides in managing hair cycle dynamics.

Author ORCID Identifier

YUN-HO KIM: 0009-0006-0381-7819

JIN KIM: 0009-0003-0700-2850

WOONG KIM: 0009-0006-0694-1591

HYUN JO: 0009-0000-8150-7011

ZHE PIAO: 0009-0003-9003-0624

YEO CHO YOON: 0000-0001-5775-5285

DOI

10.55730/1300-0152.2976

Keywords

Collagen, fibroblast growth factor 5, hair follicle, collagen tripeptide, alopecia

First Page

321

Last Page

326

Publisher

The Scientific and Technological Research Council of Türkiye (TÜBİTAK)

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

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