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Turkish Journal of Medical Sciences

Abstract

Background/aim: The etiology of hypertension in pediatric populations is complex and multifactorial, with metabolic abnormalitiesplaying a fundamental role in the pathogenesis of the condition. This study investigates the therapeutic effects of Benzyl alcohol (BA),identified through metabolomics analysis of pediatric hypertension serum, on Angiotensin II (Ang II)-induced vascular and renalinjury in murine models.

Materials and methods: Male C57BL/6 mice were used to establish a vascular remodeling model by continuous 4-week Ang II infusionusing a subcutaneous osmotic pump. Bioinformatics was used to identify target metabolites. The tail artery, common carotid arterydiastolic, and systolic pressures in mice were determined with a blood pressure monitor. Vascular structure changes were assessed withHE and Masson staining, while kidney pathology was examined using HE. Serum urea nitrogen, creatinine, and cystatin C levels weremeasured with ELISA kit.

Results: Metabolomics analysis identified metabolite BA as a potential target for hypertension management. Compared to the AngII group, BA reduced systolic blood pressure by 11.58% and diastolic blood pressure by 14.62% in the fourth week. After sodiumnitroprusside treatment, the Ang II group showed reduced vasodilation reactivity versus the control. BA significantly restored thisreactivity, unlike acetylcholine. Furthermore, BA was observed to attenuate Ang II-induced vascular mediator thickening, the mediatorto-lumen ratio, and collagen deposition. Ang II administration resulted in renal structural damage and increased concentrations of ureanitrogen, creatinine, and serum cystatin C, which was reversed by BA treatment.

Conclusion: BA exhibits potential in enhancing the vasodilatory response, vascular remodeling, and renal injury associated with Ang II.

Author ORCID Identifier

QI HUA: 0009-0002-8133-1735

ZHENYU GU: 0009-0009-8748-0345

DOI

10.55730/1300-0144.5995

Keywords

benzyl alcohol, blood vessels, kidney injury, Pediatric hypertension

First Page

509

Last Page

517

Publisher

Scientific and Technological Research Council of Türkiye (TUBITAK)

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

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