Authors: NİLÜFER GÜLMEN İMİR, AYŞEGÜL NASIRCILAR, HAKAN AKÇA, TEKİNALP GELEN, MEHMET ÖĞÜŞ, ŞEYDA KARAVELİ, OSMAN NİDAİ ÖZEŞ
Abstract: We investigated whether the activity of double-stranded RNA activated kinase (p68/PKR) is necessary for induction of IDO (indolamine 2,3 -dioxygenase) gene by IFN-\gamma. For this purpose, we planned to abrogate the function of cellular wt-PKR gene. Clones of ME180 cells expressing the dominant negative form of PKR were selected in the presence of neomycin. Then these cells were treated with IFN-\gamma and accumulation of IDO protein was determined by Western blot analysis. We found that clones expressing the dominant negative form of the PKR gene were not only resistant to IFN-\gamma-mediated killing, but were also incapable of inducing accumulation of IDO protein after IFN-\gamma treatment.
Keywords: IFN, p68/PKR, IDO, transcription
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