An Enzymatic Method for Isolating Cardiac Myocytes from Heart of Guinea-pig Used for Single-Cell and Patch-Clamp Studies

Authors: İsmail MERAL

Abstract: A method for isolating cardiac myocytes from the heart of guinea-pig is described. This method consists of perfusion of the heart with a low Ca{^2+} medium and subsequent dispersion of cells with collagenase and proteolytic enzyme. More than 80% rod-shoped, viable and quiescent cells which are suitable for electrophysiological and pharmacological experiments are obtained by this method.

Keywords: Isolated myocyte, guinea-pig, dissociation of cells.