Plasma Lipid Peroxides, Vitamin E, Superoxide Dismutase and Glutathione Alterations in Coronary Atherosclerosis

Authors: Handan AK, Nevbahar TURGAN DİNGİLOĞLU, Sara HABİF, Hakan KÜLTÜRSAY, 0ya BAYINDIR, Taner 0NAT

Abstract: The possible role of oxidant stress and antioxidant defense systems in the genesis of atherosclerosis is steadily gaining importance. From this point of view, plasma lipid peoxides (LP0), superoxide dismutase (Cu, Zn SOD), vitamin E and glutathione (GSH) levels were measured by colorimetry in 34 patients with coronary artery disease proved angiographically (7 patients with mild, 9 patients with moderate and 18 patients with severe coronary artery disease) and compared with 7 control subjects with normal coronary angiograms. Lipid peroxide concentrations were significantly higher in all three patient groups than controls (control: 1.35±0.41, mild: 1.82±0.33, moderate: 1.86±0.59 and severe: 2.55±0.72 nmol/ml, mean±SD, P<0.05; p<0.05 and p<0.001, respectively). Plasma vitamin E levels were significantly higher in patients with moderate (mean+-SD, mg/dl: 0.61+-0.23, p<0.05) and severe (0.36+-0.19, p<0.001) coronary artery disease when compared with controls (1.09+-0.39). Plasma Cu, Zn superoxide dismutase activities were significantly different from the controls (mean+-SD, ng/ml: 169.6+-68.39) in the moderate (109.3+-40.2, p<0.001) and severe CAD (68.3+-34.6, p<0.001). GSH consentrations difeered only in the svere group (mean:+-SD, mg/dl erytrocyte: 32.13+-4.13 p<0.05) when compared with the controls (37.3+-5.16). Overall three were significant negative correlations between lipid peroxides and vitamin E(r_s=-0.603, p<0.0001), between lipid peroxides and vitamin SOD (r_s=-0.481, p<0.05) and a positive correlation between vitamin E and SOD (r_s=0.549, p<0.001). The results of the present study showed that plasma lipid peroxides were increased and antioxidant defense systems were decreased in coronary heart disease and that they might be used as determinants in the assessment of the severity of CAD.

Keywords: Lipid peroxides, vitamin E, Cu, Zn-superoxide dismutase, glutathione, coronary artery disease