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Turkish Journal of Chemistry

Abstract

Hypochlorous acid (HOCl) is a potent nonradical oxidant involved in various physiological processes, particularly within the human immune system. In this study, we introduce a novel, rapid, and highly efficient fluorometric method for the detection of HOCl. The method utilizes a near-infrared (NIR)-based fluorescent probe, NIR-QBH, which is characterized by its high sensitivity and chemical stability. NIR-QBH, containing olefinic C=C bonds, exhibits strong NIR emission at 660 nm (λex = 618 nm). The detection mechanism relies on the oxidation of the C=C bond in the NIR-QBH structure by HOCl, resulting in the formation of non-fluorescent products. With a detection limit of 0.23 μM, the probe demonstrates a fast response time of 4 min. Glutathione (GSH), an essential biothiol, was employed as a reference HOCl scavenger, and its HOCl scavenging activity was evaluated with an IC50 value of 8.97 μM. Furthermore, the developed fluorometric assay was successfully applied for the detection of HOCl in fetal bovine serum (FBS) and aqueous solutions.

Author ORCID Identifier

AYTEN IBRAHIMOVA: 0000-0002-9659-5218

AYŞE ÖNEM: 0000-0002-6201-6740

MEHMET ALTUN: 0000-0003-2541-0623

EMİN YEŞİL: 0000-0001-8559-1951

ASLI BAYSAL: 0000-0002-0178-7808

HASAN SAYGIN: 0000-0003-2479-7635

AHU SOYOCAK: 0000-0003-0999-2774

MUSTAFA ÖZYÜREK: 0000-0001-5426-9775

DOI

10.55730/1300-0527.3725

Keywords

Hypochlorous acid, NIR, fluorogenic probe, GSH, cytotoxicity

First Page

241

Last Page

253

Publisher

Scientific and Technological Research Council of Türkiye (TUBITAK)

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

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