Flowerlike hybrid horseradish peroxidase nanobiocatalyst for the polymerization of guaiacol


Abstract: In this study, the catalytic activity and stability of flowerlike hybrid horseradish peroxidase (HRP) nanobiocatalyst (HRP-Cu$^{2+}$) obtained from Cu$^{2+}$ ions and HRP enzyme in the polymerization reaction of guaiacol were analyzed. We demonstrated that HRP-Cu$^{2+}$ and hydrogen peroxide (H$_{2}$O$_{2}$) initiator showed significantly increased catalytic activity and stability on the polymerization of guaiacol compared to that of free HRP enzyme. Poly(guaiacol) was observed with quite high yields (88%) and molecular weights (38,000 g/mol) under pH 7.4 phosphate-buffered saline (PBS) conditions at 60 °C with 5 weight% of HRP-Cu$^{2+}$ loading. HRP-Cu$^{2+}$ also shows very high thermal stability and works even at 70 °C reaction temperature; free HRP enzyme denatures at that temperature. Furthermore, HRP-Cu$^{2+}$ provided considerable repeated use and showed some degree of catalytic activity, even after the fourth recycle, in the polymerization of guaiacol.

Keywords: Horseradish peroxidase, hybrid nanoflowers, polymerization, guaiacol

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