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Turkish Journal of Botany

Abstract

The widespread utilisation of deep sequencing has dramatically enhanced and revolutionised our understanding of virus diversity, particularly in complex environments such as soil and biological samples. This research explored mycovirus sequence analyses in the ectomycorrhizal fungus Amanita vidua, utilising deep sequencing alongside total double-stranded RNA (dsRNA) extractions. Our investigation unveiled a unique member of the proposed family, Narliviridae, designated as Amanita vidua narlivirus 1 (AvNV1), in an A. vidua specimen. AvNV1, classified under Amabiliviricetes, possesses a distinct genome structure and sequence attributes. T horough analyses indicated that AvNV1 exhibits sequence similarities with other mycoviruses from the proposed family Narliviridae and forms a cluster within an as-yet-unclassified genus. The identification of AvNV1 contributes to the genetic variety of mycoviruses in the Amabiliviricetes category, providing insights into their evolutionary trajectory. In conclusion, this study underscores the significance of delving into viral diversity in noncultivatable fungi, filling in gaps in our understanding of mycovirus ecology. Detecting a new narlivirus expands our knowledge of mycovirus diversity and evolution, illustrating the necessity for ongoing research into viral communities in noncultivatable fungi.

Author ORCID Identifier

ILGAZ AKATA: 0000-0002-1731-1302

EDA KUMRU: 0009-0000-7417-6197

GÜLCE EDİŞ: 0000-0001-7038-9865

ERTUĞRUL KAYA: 0000-0003-0081-682X

ERGİN ŞAHİN: 0000-0003-1711-738X

DOI

10.55730/1300-008X.2857

Keywords

Amabiliviricetes, Amanita vidua, ectomycorrhizae, Mycovirus, narlivirus

First Page

220

Last Page

228

Publisher

The Scientific and Technological Research Council of Türkiye (TÜBİTAK)

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

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Botany Commons

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