In this work, 10 sunflower ( Helianthus annuus L.) genotypes were evaluated for their suitability for Agrobacterium tumefaciens-mediated gene transfer based on detection of a reported gene expression (GUS). A number of factors including the type of shoot-tip explant (split vs. intact), bacterial strain/binary vector combinations and wounding intact shoot-tips by particle bombardment were examined. Genotypes showed significant differences in their transformation efficiencies ranging from 0.0 to 82.7% GUS positive explants, hybrid genotypes being more responsive to Agrobacterium infection than inbred lines. Use of split shoot-tip explants did not increase the transformation efficiency over intact explants but the AGL-1/pKIWI strain/vector combination was more effective than the LBA4404/pTOK233 combination. Wounding explants by particle bombardment prior to inoculations with Agrobacterium had no positive effect on transformation.
Sunflower, gene transfer, Agrobacterium tumefaciens, genotypes.
GÜREL, EKREM and KAZAN, KEMAL (1999) "Evaluation of Various Sunflower ( Helianthus annuus L.) Genotypes for Agrobacterium tumefaciens-mediated Gene Transfer," Turkish Journal of Botany: Vol. 23: No. 3, Article 2. Available at: https://journals.tubitak.gov.tr/botany/vol23/iss3/2