Isolation, Identification and Toxigenic Tests of Aeromonas hydrophila from Several Sources in Izmir


Abstract: A total of 43 samples (4 sea water, 5 water, 4 river water, 10 oyster and 10 fish samples) from Izmir were examined for the presence of Aeromonas hydrophila. Alkaline peptone Water (APW) for the environmental samples and Trypticase Soy Broth with Ampicillin and Toluidine Blue (TSBA) for the food samples were used for enrichment of the samples. In isolation, 5 different selective media were used: DNase Agar with Toluidine blue and Ampicillin (DNTA), Aeromonas Agar (AA), Rimler Shotts Agar (RSA), mA Agar and Aeromonas Mediums Base (AMB). Identification of the isolates was performed according to Bergey's Manual of Determinative Bacteriology (1984) and 9 isolates were identified as A. hydrophila. The enterotoxin production of 9 isolates was investigated by the suckling mouse assay and 8 of the 9 isolates were found to be enterotoxigenic.

Keywords: Aeromanas hydrophila, AMB, suckling mouse assay

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