Authors: ÖZLEM EREN KIRAN, UĞUR ÇÖMLEKÇİOĞLU, BURHAN ARIKAN
Abstract: The amylase producer thermophilic Bacillus sp. K-12 was isolated from soil samples from Zeytinli hot spring in Kahramanmaraş. Enzyme synthesis occurred at 20-55 ºC with an optimum of 42 ºC. There was a slight variation in amylase synthesis within the pH range 4.5-10.5. Effects of various carbon sources and chemicals on a-amylase production were examined and maximum a-amylase production was obtained in a medium containing 1% starch in 60 h. MnSO_4, ZnSO_4 and EDTA inhibited a-amylase production of Bacillus sp. K-12.
Keywords: Thermostable, Amylase, Bacillus
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