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Turkish Journal of Agriculture and Forestry

Abstract

The common name of a wild grass native to Pakistan with both edible and medicinal properties is Saccharum munja Roxb. (S. munja). Prior research has investigated various plant species for the green biosynthesis of nanoparticles to circumvent issues related to their physical and chemical production. This study presents the first evidence of synthesizing cobalt oxide nanoparticles (Co3O4NPs) using S. munja leaf extract (SMLE) as both a reducing and a capping agent. This study investigated their ability to induce in vitro callus formation in Dianthus caryophyllus L. (carnation). In this study, Co3O4NPs were synthesized using varying quantities of SMLE to achieve increased biocompatibility, and the presence of steroids, saponins, tannins, flavonoids, and proteins was confirmed by the phytochemical screening of SMLE. A UV-Vis spectrophotometer displaying absorbance wavelengths from 570 to 610 nm was used to verify the presence of Co3O4NPs. The experiment using 3.24 g of CoCl2 dissolved in 50 mL of distilled water with 5 mL of SMLE (distilled water) and 5 mL of SMLE (ethanol) produced the optimal absorption peak, according to the data. When Co3O4NPs (0, 10, 15, and 20 mg/L) were added to carnation for callus induction, 15 mg/L produced the highest results, followed by 20, 10, and 0 mg/L. Among the tested doses, 15 mg/L demonstrated the most significant results in terms of callus fresh weight, callus size, and callus proliferation. In conclusion, Co3O4NPs were successfully biosynthesized from SMLE and played a significant role in callus development and proliferation in carnation. Being an inexpensive weed, SMLE provides valuable insight into the biological production of eco-friendly Co3O4NPs and their commercial application in tissue culture research involving related plant species.

Author ORCID Identifier

ISHTIAQ AHMAD: 0000-0002-9735-6542

SAMEED ARSHAD CHAUDHRY: 0009-0009-4231-7990

MARYAM: 0000-0002-9095-8612

AYSHA ARIF CHAHEL: 0000-0001-5261-4301

RIAZ AHMAD: 0000-0002-4383-8335

MEHMET RAMAZAN BOZHÜYÜK: 0000-0001-5021-6019

İBRAHİM BOZKURT: 0009-0000-4509-8855

MAGDA ABDEL-RAHMAN: 0000-0001-8667-8661

KONSTANTIN KOROLEV: 0000-0001-9595-3493

DOI

10.55730/1300-011X.3286

Keywords

Callogenesis, cobalt oxide nanomaterials, eco-friendly synthesis, in vitro culture

First Page

558

Last Page

566

Publisher

The Scientific and Technological Research Council of Türkiye (TÜBİTAK)

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

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