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Turkish Journal of Agriculture and Forestry

Abstract

Commiphora myrrha grows in various places of the world and is used for many purposes. The objectives of this study were to examine the biological influences of C. myrrha resin extract (MRE) alone or with its biogenic nanosilver on microbial, immune, and cancer cell growth. C. myrrha resin was boiled to prepare the MRE. Functional groups in the MRE were explored using Fourier-transform infrared spectroscopy (FTIR). MRE was used to synthesize nanosilver that was characterized using an ultraviolet-visible (UV-Vis)-spectrophotometer and X-ray diffraction. In vivo experiments included the untreated group (G1) and MRE-orally treated group (G2). Cancer cell lines, HCT-116 (colon) and MCF-7 (breast) were used to test the anticancer potential of the MRE and MRE + nanosilver. Immunomodulatory potential of the MRE and MRE + nanosilver were tested using rat splenic cells of normal and MRE-treated rats. Physiological effects on vital organs (liver and kidneys) were tested. The results revealed that MRE contained many functional groups. The MRE created a nanosilver with a size of 18.9 nm. MRE showed no antibacterial activity, while MRE + nanosilver showed higher activity at 1000 μg/mL (12.14 ± 0.25 mm). MRE and MRE + nanosilver showed stimulatory effects on the HCT-116 cells (110%–185%), while both preparations diminished MCF-7 cell growth at 250–500 μg/mL. The MRE showed no side effects on the liver and kidneys after oral administration for 30 days. It also induced the proliferation of untreated rat splenic cells with an average percentage of 110%–200%, and displayed good immune boosting effects as it stimulated untreated rat splenic cells (110%–200%). MRE and MRE + nanosilver may serve as druggable targets for CD4+ T cell infiltration, improving clinical outcomes in luminal breast cancer patients via targeting tumor suppressor protein p53 (TP53). Continuous oral uptake of MRE created a state of oral tolerance. In conclusion, MRE and MRE + nanosilver potentially mitigated the growth of cancer cells. Additionally, MRE showed immune-boosting effects and no side effects on vital organs after oral administration.

Author ORCID Identifier

MOHAMMAD Y. ALSHAHRANI: 0000-0002-7096-0221

ESSAM H. IBRAHIM: 0000-0003-0130-2257

ABEER S. ALAHMARI: 0000-0001-6310-7815

MONA KILANY: 0000-0002-9538-6799

RAMADAN TAHA: 0000-0003-3911-1747

HAITHAM I. El-MEKKAWY: 0000-0001-9363-283X

AMR AHMED El-ARABEY: 0000-0003-0420-7191

AHMED EZZAT AHMED: 0000-0002-4532-0636

DOI

10.55730/1300-011X.3285

Keywords

Colon cancer (HCT-116) cell line, breast cancer (MCF-7) cell line, TP53, CD4+ T cells, splenic cells, oral tolerance

First Page

543

Last Page

557

Publisher

The Scientific and Technological Research Council of Türkiye (TÜBİTAK)

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

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