Effect of Colchicine Treatment with Different Doses and Periods on in vitro Chromosome Doublication in Haploid Watermelon

Authors: Nebahat SARI, Kazım ABAK

Abstract: Differents colchicine doses (0.5 % and 1 %) and treatment periods (1, 2, 4 and 6 hours) were tested in vitro conditions in order to obtain dihaploid lines from parthenogenetic haploid plants which achieved by irradiated pollen. Treatments were made to 3-4 weeks age in vitro microcuttings of Sugar Baby, Halep Karasi and Crimson Sweet varieties. 64 percent of the plants treated with 0.5 % colchicine stayed alive and 56 % of the plants were developed into a new plant. At the doses of 1% the rate of alive and growing plant decrease to 40 %. As for as the treatment periods were considered the highest death were observed from the 6 hours treatment. Results showed that in order to obtain dihaploid lines by colchicine treatment for either 4 hours at 0.5 % or 2 hours at 1 % for microcuttings were the most favorable condition. Higher doses and long period treatment resulted in death plant and olw doses and short period treatment didn2t change the ploidy level.


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