Authors: TAO LAN, YUANLIN DUAN, BIN WANG, YUANCHANG ZHOU, WEIREN WU
Abstract: Na^+/H^+ antiporters have been suggested to play important roles in salt tolerance in plants. English cordgrass (Spartina anglica) is a gramineous halophyte with very strong salt tolerance. It possesses salt glands in its stems and leaves, through which excess salt can be excreted. In this study, a vacuolar Na^+/H^+ antiporter homologous gene (temporarily named SaNHX1) was isolated from Spartina anglica by RT-PCR and rapid amplification of cDNA ends (RACE). Southern blot analysis suggested that there might be 2 or 3 copies of the vacuolar Na^+/H^+ antiporter genes in the English cordgrass genome. Northern blot analysis showed that the expression of vacuolar Na^+/H^+ antiporter genes in English cordgrass is induced by salt stress. Overexpression of SaNHX1 driven by constitutive promoter Ubi-1 in rice significantly enhanced the salt tolerance of transgenic plants, validating the function of SaNHX1 and suggesting its value for the genetic improvement of salt tolerance in plants.
Keywords: Halophyte, salt tolerance, SaNHX1, Spartina anglica, transgenic rice
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