A comparison of skeletal muscle protein bands in Pelophylax ridibundus (Pallas, 1771) and Pelophylax caralitanus (Arıkan, 1988) populations in Turkey using SDS-PAGE


Abstract: In this study, skeletal muscle protein bands of Pelophylax ridibundus specimens from 31 populations inhabiting all geographic regions of Turkey and Pelophylax caralitanus specimens from the Isparta (Lake Eğirdir) population were compared using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). In total, 270 adult (134 females, 136 males) P. ridibundus specimens and 12 adult (5 females, 7 males) P. caralitanus specimens were used in SDS-PAGE characterization. The first group (31 different populations of P. ridibundus) had 27 homologous protein bands whereas the second group (1 population of P. caralitanus from Lake Eğirdir) had 28 bands. It was shown that the second group had one additional and unique band. The results of the present study show that specimens of the P. ridibundus and P. caralitanus populations in Turkey are different in terms of the total number of skeletal muscle protein bands.

Keywords: Skeletal muscle, SDS-PAGE, P. ridibundus, P. caralitanus, protein band

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