Authors: SAMAD KHAGHANINIA, SEYED ABOLGASEM MOHAMMADI, ALI MORAD SARAFRAZI, KARIM HADDAD IRANI NEJAD
Abstract: In order to study population structure in the codling moth (Cydia pomonella L.) using RAPD markers, 13 geographical populations from northwestern Iran were collected during 2003 and 2004. Genomic DNA was extracted from 10 overwintering larvae of each population. Out of 60 tested primers, 18 amplified 236 polymorphic bands. The total number of bands in the population varied from 169 to 206 in the Mughan and Zunuz populations, respectively. Within-population genetic diversity, based on Nei's genetic index, ranged from 0.228 to 0.281 for the Shabestar and Zunuz populations, respectively. An analysis of the molecular variance revealed significant differences within and between population variance. Between-population variation accounted for 14.44% and within-population variation accounted for 85.56% of the total molecular variance. Cluster analysis based on molecular data assigned the studied codling moth populations to 2 groups. In this grouping, Group 1 consisted of the Mughan population only. The maximum and minimum genetic distances were observed between the Mughan-Ahar and Shabestar-Mahabad populations, respectively. Canonical correlation analysis showed significant association between RAPD markers and the latitude of the studied regions. A principal coordinate analysis showed high discrimination between geographic populations and confirmed the results of the cluster analysis. A significant correlation was found between genetic and geographic distance matrices as revealed by the Mantel test.
Keywords: Cydia pomonella, molecular data, population variation, RAPD
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