Authors: TEODORA M. GEORGIEVA, DIMITRINKA S. ZAPRYANOVA, EVGENIYA V. DISHLYANOVA, STOYAN I. TANEV, IVAN P. GEORGIEV, MARIA I. ANDONOVA, IVAN N. KANELOV, LAZARIN V. LAZAROV, PETYA I. KOLEVA
Abstract: The present study provides the results from a comparative study of the 3 commonly used methods for total protein (TP) measurement. The experiments were carried out with 6 dogs (4-7 year-old, weighing 12.8 ± 1.4 kg). Five blood samples were obtained by saphena venepuncture from all dogs, during the time course of the experimentally induced infection with Staphylococcus intermedius, administered subcutaneously at a dose rate of 5 ml of 1.10^9 CFU/ml within 14 days. TP concentration was measured by 2 macro protein techniques - biuret method (commonly used) and method of Lowry, and a modified version of biuret method (micro protein technique), suggested by Popov. Serum TP concentration determined by the method of Lowry was significantly (P < 0.001) higher than the ones obtained by standard biuret and Popov´s methods. The mean differences between TP values obtained by standard biuret technique and Lowry´s method and, Lowry´s and Popov´s method were 18.6 g/l and 23.5 g/l, respectively. There was no statistically significant difference between standard biuret method and its modified version suggested by Popov.
Keywords: Total protein concentration, dogs, biuret method, method of Lowry, modification biuret technique by Popov
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