Detection of Antibodies Produced in Quails (Coturnix coturnix japonica) against Mycoplasma gallisepticum with Different Serological Tests


Abstract: The objectives of this study were to isolate and identify Mycoplasma gallisepticum in quails with clinical and pathological signs of chronic respiratory disease (CRD), and to compare the sensitivity and specificity of commonly used serological tests that are used for the detection of M. gallisepticum antibodies. The study included 20 Japanese quails 10-weeks old with respiratory distress, nasal discharge, mortality and swollen infraorbital sinuses. There was no bacteriological agent isolated. All of the 20 sera samples obtained from the quails were evaluated with AGID, RSA, HI, and ELISA; 2 (10%), 5 (25%), 10 (50%), and 17 (85%) sera samples were positive, respectively. In conclusion, for detecting M. gallisepticum antibodies in quails the use of ELISA, which is a very sensitive test, together with other conventional tests was beneficial.

Keywords: Mycoplasma gallisepticum antibodies, serologic tests, quail

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