Authors: SÜHEYLA TÜRKYILMAZ, SEZA ESKİİZMİRLİLER
Abstract: In this study, the production of slime factor in 180 Staphylococcus spp. (90 coagulase-positive Staphylococcus (CoPS) and 90 coagulase-negative Staphylococcus (CoNS)) isolated from various animal clinical specimens was investigated with Congo Red Agar (CRA), Microplate (MP), and Standard Tube (ST) tests and the results were compared to each other. The rate of production of slime factor in all of the Staphylococcus spp. investigated with CRA, MP, and ST tests were 61.1%, 55.5%, and 50.5%, respectively. The existence of Staphylococcus spp. resistance against various antibiotics was also determined by the agar disk diffusion method. The percentage of resistance against penicillin, methicillin, ampicillin, and gentamycin in slime-producing (SP) Staphylococcus spp. was 49.0%, 24.5%, 23.6%, and 13.6%, respectively, whereas for non-slime-producing (NSP) strains it was 42.9%, 15.7%, 14.2%, and 12.9%, respectively. The comparison of SP strains with NSP strains revealed that SP strains had more resistance to those antibiotics. It was determined that all of the Staphylococcus spp. were susceptible to vancomycin. The results observed from this study showed that there was no statistically significant difference between the tests applied (CRA, MP, and ST tests; X^2 = 0.28). In conclusion, the CRA test could be used for the detection of slime production in Staphylococcus spp. because it is reliable and practical.
Keywords: Slime production, detection techniques, Staphylococcus spp.
Full Text: PDF