A Comparative Study of Detection of Bordetella avium Antibodies in Turkeys by ELISA, SPAT, and AGID Test


Abstract: The aims of this study were to develop a serum plate agglutination test (SPAT) antigen and agar gel immunodiffusion (AGID) test antigen for the serological detection of turkeys that have been exposed to Bordetella avium; to compare the sensitivity of commercial enzyme-linked immunosorbent assay (ELISA) with SPAT, and AGID test, and to survey B. avium antibodies in turkey flocks in Aydın, Turkey. For these purposes, serum samples collected from 300 turkeys were examined by ELISA, SPAT, and AGID test. The seroprevalence was 44%, 28%, and 15% for ELISA, SPAT, and AGID test, respectively. This study revealed that ELISA was the most sensitive test for the detection of B. avium antibodies; however, it was determined that SPAT and AGID test can also be used in screening for B. avium antibodies.

Keywords: Bordetella avium, ELISA, SPAT, AGID

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