Authors: UÇKUN SAİT UÇAN, LEYLA AÇIK, AYTEN ÇELEBİ, OSMAN ERGANİŞ, EMİNE ARSLAN
Abstract: In this study, a total of 30 Escherichia coli isolates obtained from milk samples of dairy cows suffering from subclinical mastitis in Konya, Turkey were typed according to plasmids and protein patterns. Agarose gel electrophoresis and sodium doedecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) methods were used to identify plasmids and whole-cell protein profiles. Of these two methods, SDS-PAGE typing proved to be more discriminate for typing the isolates.
Keywords: SDS-PAGE, plasmid, mastitis, E. coli, bovine
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