The Effect of Acute Fluoride Poisoning on Nitric Oxide and Methemoglobin Formation in the Guinea pig


Abstract: To study the effect of acute fluoride poisoning on nitric oxide and methemoglobin formation, 250 mg/kg bw sodium fluoride was applied alone and verapamil was applied together with fluoride. Blood nitric oxide (Griess reaction) and calcium levels; hemoglobin, methemoglobin and hematocrit values; and erythrocyte counts were determined and compared with those of the controls. After the fluoride application it was found that there was a relative relationship between the increase in nitric oxide and methemoglobin levels and the decrease in calcium, hemoglobin and hematocrit levels and erythrocyte count. It was concluded that the increase seen in blood nitric oxide levels as a result of the ionophore effect of fluoride could come from cNOS, as that increase is related to the decrease in calcium amount.

Keywords: Fluoride poisoning, nitric oxide, methemoglobin, guinea pig

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