Immunoblot Analysis of Campylobacters of Chickens


Abstract: The aim of this study was to determine immunogenic structures by immunoblotting in thermophilic Campylobacter strains isolated from chickens. The bands obtained from C. jejuni strains were found to be distributed between 10.8 and 116.5 kD by SDS-PAGE analysis of whole cell proteins. The bands 78, 63, 52, 48.5, 43.5, 29.8, 25.6, 22, 18.5, 17.2 and 16.5 kD were detected as the major common bands in all C. jejuni strains. Electrophoretic analysis of C. coli strains revealed that protein band profiles ranged between 10.3 and 118 kD, and 76, 63-62, 52, 48.5, 44 and 30.5 kD bands were all major bands. Common antigenic structures with 67, 63, 43 and 29-31 kD were observed in the immunoblot analysis (Western blotting) of C. jejuni and C. coli strains. In addition, specific bands with 27 kD and 38 kD were detected in C. jejuni and C. coli strains, respectively. There was no difference in the cross immunoblot analysis carried out with immune sera prepared against C. jejuni and C. coli strains. In conclusion, protein profiles and immunogenic structures obtained by electrophoretic analysis of C. jejuni and C. coli strains were found to be similar.

Keywords: Campylobacter, immunoblotting, SDS-PAGE, chicken

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