Authors: AYKUT ÖZKUL, İHSAN KELEŞ, TANER KARAOĞLU, MEHMET ÇABALAR, İBRAHİM BURGU
Abstract: In this study, the detection of canine parvovirus (CPV) in a fecal sample from a dog with enteritis was performed for the first time using the polymerase chain reaction (PCR) in Turkey. The final PCR product was analyzed using the restriction fragment length polymorphysm (RFLP) technique. RFLP analysis using Apa LI and Eco RV restriction endonucleases revealed homology in the nucleotide sequence in at least the VP2 coding region of the virus DNAs detected in the fecal specimen and prepared from attenuated vaccine virus as a positive control.
Keywords: Canine parvovirus, diagnosis, PCR, RFLP
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