Immunohistochemical Localization of some Peptides in the Gastrointestinal Tract Mucosa of Pike-perch (Stizostedion lucioperca L., 1758)


Abstract: In this study, our aim was to determine the distribution of specific immunoreactivities in some regions of the digestive tract of the pike-perch (Stizostedion lucioperca) against the antisera Gastrin, Serotonin (5 HT), and Cholecystokinin (CCK). The samples were taken from the stomach, intestine and pyloric caeca and the Avidin-Biotin-Immunoperoxidase method was applied. At the end of this application the immunoreactivities against all antisera were observed as intensively in the cells of the gland and less in the cells of Lamina epithelialis. The most intensive regions of immunoreactive cells were determined as the fundus, pylorus, anterior intestine and pyloric caeca. Immunoreactivity was determined in the nerves belonging to the myenteric plexus and muscle tissue and also ganglion cells against some antisera to be less and poorer except cells in the mentioned regions.

Keywords: Pike-perch, Gastrointestinal tract, Endocrine cell, Peptide, Immunohistochemistry

Full Text: PDF