Authors: RIFKI HAZIROĞLU, JALE ERDEĞER, M. YAVUZ GÜLBAHAR, OĞUZ KUL, MURAT YILDIRIM
Abstract: One hundred pneumonic lungs from calves were examined by an immunoper oxidase technique using avidin-biotin- per oxidase complex method in formalin-fixed, paraffin-embedded sections for Haemophilus somnus. In the histological examination of tissue sections, strong positive reactions against H. somnus were detected in 14% of the lungs. H. somnus antigens were observed at the surface and/or within the epithelial cells, macrophages, necrotic alveolar walls, alveolar -bronchial/br onchiolar exudates, fibrins, distended interlobular septa and perivascular areas. In addition, dense antigen accumulations were detected in the necrotic macrophages and neutrophils.
Keywords: Haemophilus somnus, Immunoper oxidase, Pneumoniae.
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