Detection of Antibodies to Ehrlichia risticii in Horses

Authors: Seyyal AK, Nuri TURAN, Ahmet MİNBAY, Sukanta K. DUTTA

Abstract: In this study, presence of antibodies to Ehrlichia risticii in the sera of 100 throughbreed horses brought to Turkey Jockey Club Stud from different regions was investigated by indirect fluorescent antibody (IFA), western immunoblotting, indirect enzyme linked immunosorbent assay (ELISA) and competetive ELISA (CELISA) using monoclonal antibody. Ten sera gave various degrees of flourescence on IFA test. The sera giving fluorescence were sent to University of Maryland and the test was repeated. The results were similar. Five sera from those positives were also tested on western immunoblotting. Amongst those, only one serum reacted with one of the 9 surface antigens of E. risticii. This serum reacted with an antigen on 38 kD region on the firs test and with 28 kD region on the second. Therefore, the reaction obtained by this serum on western immunoblotting was considered non-specific as the result were inconsistent. No antibody to E. risticii was detected in any of 100 horse sera by CELISA and indirect ELISA. In conclusion, 10 sera of 100 horse sera gave fluorescence on IFA test for E. risticii. However, these were negative on western immunoblotting, indirect ELISA and CELISA using monoclonal.

Keywords: Ehrlichia risticii, horses, IFA, CELISA, ELISA, western immunoblotting.

Full Text: PDF