Authors: KORAY TEKİN, ALİ DAŞKIN
Abstract: Use of computer-assisted sperm analyzers (CASAs) to evaluate sperm fertilizing ability has great importance for the artificial insemination industry. For motility evaluation, different CASA systems and diluents are being used. These assays are poor in predicting sperm fertility, because only the samples with markedly poor quality can be detected. However, there is not a unitary extender worldwide. Therefore, we aimed to evaluate the postthaw kinetic parameters of curvilinear velocity (VCL), straight-line velocity (VSL), and average-path velocity (VAP) with two different extenders (skim milk, SM; Tris, TS). Semen was collected twice a week with an artificial vagina from four adult Norduz bucks. The ejaculates having normospermic quality were split into two equal aliquots and frozen. Sperm motility (%), VCL, VSL, VAP, viability (%), and abnormal spermatozoa (%) were evaluated. Regarding the abnormal spermatozoa rates and viability, percentages of motility were significantly higher in SM extender (P < 0.05), and VCL, VSL, and VAP rates were found higher with TS. In conclusion, according to the in vitro findings of this study, motility rates are more favorable in SM extenders than TS, and spermatozoa swim significantly faster in TS extender compared to SM.
Keywords: Computer-assisted sperm analyzer, cryopreservation, motility, Norduz goat, velocity
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