Authors: MEI JIN, XIUNA LI, JUN PIAO, YANGLE QU, YIMENG WANG, JINGAI PIAO, MINGXING CHU
Abstract: This study aimed to characterize the expression the GPRC5D gene in the skin of the Liaoning Cashmere goat. Initially, the GPRC5D gene sequence was subjected to bioinformatics analysis. Second, semiquantitative RT-PCR was used to detect GPRC5D gene expression in the tissues. Third, in situ hybridization was used to locate GPRC5D gene expression in the primary and secondary hair follicles. Functional site analysis showed that GPRC5D has 7 transmembrane domains. RT-PCR showed that GPRC5D was expressed only in the skin. In situ hybridization showed that GPRC5D was expressed in the inner root sheath only. We speculated that GPRC5D may regulate cashmere growth.
Keywords: GPRC5D, bioinformatics, RT-PCR, in situ hybridization
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