Authors: Haydar BAĞIŞ, Serhat PAPUÇÇUOĞLU
Abstract: This paper describes attempts to identify parameters that are critical for succesful gene transfer into the germ line of mice by microinjection of foreign DNA into fertilized eggs. In the first stage of the study, 1470 zygotes were obtained and 1269 (86%) were microinjected with a human growth hormone gene construct. Of these zygotes, 769 (60%) proved to be alive after the injection, and 420 of them were transfered into the pseudopregnant recipients (54%). As a result, 70 offsprings were obtained, and out of these 3 (4%) proved to be transgenic. In the second stage of the study, 1246 zygotes were obtained and 1020 (81%) were microinjected with a human p53 gene construct. Of these zygotes 601 (58%) proved to be alive after the injection and 400 (66%) of them were transfered into pseudopregnant recipients. As a result, 105 (26%) offsprings were obtained, and out of these, 6 (5%) proved to be transgenic. Comparison of the ratios of transgenic mice in both groups showed no significant difference (P>0.05).
Keywords: Mouse embryo, DNA, microinjection, embryo transfer