Authors: ALFREDO GARCIA, REMIGIO MARTINEZ, LOURDES GARCIA, JOSE MANUEL BENITEZ-MEDINA, DAVID RISCO, WALDO LUIS GARCIA, JOAQUIN REY, JUAN MANUEL ALONSO, ANGELI KODJO
Abstract: Sixty-five rats (Rattus norvegicus) and 5 mice (Mus musculus) were trapped from 8 outdoor farms and the presence of Shiga toxin-producing Escherichia coli was not detected in any individual. Nevertheless, using the microscopic agglutination test, it was found that 20% (13/65) of rat serum samples were positive for anti-Leptospira antibodies, and DNA of pathogenic leptospires was detected in 3.07% (2/65) of the rat kidneys by real-time polymerase chain reaction. The most common infecting serovar was Castellonis (7.69%), followed by Canicola (6.15%) and Mangus (6.15%). According to these results, rats from outdoor farms may be considered a reservoir host for Leptospira in the study area.
Keywords: Rodents, Shiga-toxin producing Escherichia coli, Leptospira spp., outdoor farms
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