Authors: JIHONG ZHANG, SHIJUN ZHENG, TANGMING ZHU, SIGUO LIU, RUSONG LI, TING ZHU, SUWEN HUANG, JIANFENG WANG, JIANBO NI, XIULING ZHAO, JING SUN, SHAOTANG HUANG
Abstract: A rapid and effective polymerase chain reaction (PCR) test, specific for the detection of Mycobacterium bovis and Mycobacterium avium subsp. paratuberculosis in invasive flies, was developed. This duplex PCR technique was specifically designed to amplify the sequences of IS6110 of M. bovis and IS900 of M. avium subsp. paratuberculosis. The results of the interference test, simulated contamination test, and several other experiments indicated that this technique had high stability, specificity, and sensitivity with detection limits at 92.98 pg of M. bovis and 110.27 pg of M. avium subsp. paratuberculosis. Therefore, this duplex PCR test is a rapid and effective technique to simultaneously detect M. bovis and M. avium subsp. paratuberculosis.
Keywords: Fly, Mycobacterium bovis, Mycobacterium avium subsp. paratuberculosis, duplex PCR
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