Authors: MICHAIL PASKALEV, SVETOZAR KRASTEV
Abstract: The aim of the present study was to monitor serum tartrate-resistant acid phosphatase (TRAP) and C-terminal telopeptide fragments of type I collagen (CTX) over time in canine experimental osteotomies fixed using 2 osteosynthesis techniques, to determine the relevance of both markers in monitoring bone healing, and to investigate the influence of the 2 osteosynthesis techniques on their concentrations. Transperiosteal osteotomy of the diaphyses of the right tibia and fibula was performed in 12 dogs. The dogs were then randomly assigned to 1 of 2 groups: group 1 (6 dogs) underwent osteotomy fixed with intramedullary osteosynthesis (IMO) and group 2 (6 dogs) underwent osteotomy fixed with a plate (plate osteosynthesis [PLO]). Craniocaudal radiographs were obtained immediately after osteosynthesis, 2 weeks post-surgery and 1, 2, 3, 4, 5, and 6 months post-surgery. The evaluation of bone resorption was performed visually using a 4-grade scoring system. At the same time points, venous blood was sampled for determination of tartrate-resistant acid phosphatase and the concentration of C-terminal telopeptide fragments of type I collagen. Radiologically visible bone resorption was characterized by 2 peaks. The 1st peak occurred by the end of the 2nd postoperative week with both osteosynthesis methods (P < 0.001). The 2nd peak began by the end of the 4th postoperative month and persisted until the end of the experiment. Serum TRAP was not a reliable marker of bone resorption during the study period. CTX concentrations increased considerably in both groups by the end of the 1st postoperative month (P < 0.05), decreased by the 3rd postoperative month, and increased again by the end of the 5th postoperative month. CTX, therefore, could be used to monitor normal bone healing.
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