Authors: NIKOLINA VELIZAROVA RUSENOVA, ANELIYA MILANOVA HARITOVA, PARVAN RUSENOV PARVANOV
Abstract: A reference strain Escherichia coli ATCC 25922, sensitive and resistant strains of E. coli, isolated from chickens with colibacillosis were tested. The aim of the present study was to determine the effect of modulators of ABC efflux transporters in eukaryotic cells cyclosporine A and quercetine on MIC values of danofloxacin mesylate. MIC values of danofloxacine mesylate against the chosen strains were determined with or without modulators at concentrations of 25 \mu M, 5 \mu M, 1 \mu M, and 0.2 \mu M by macro broth dilution method. Results show that in the presence of cyclosporine A, MIC values decreased from 2-fold to 16-fold in the investigated strains of E. coli. Combination of danofloxacin mesylate and quercetine resulted in an increase of MIC values. The observed changes in the values of MICs suggest participation of ATP-dependent efflux of danofloxacin mesylate from bacterial cells.
Keywords: Danofloxacin mesylate, cyclosporine A, quercetine, Escherichia coli
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